Exploratory Study on 35 kDa Hyaluronan with Microneedling for Skin Concerns: A Series of 16 Cases
Purpose: This study aims to evaluate the short-term effectiveness and safety of 35 kDa low molecular weight Hyaluronan (HA35) combined with negative pressure microneedling technology in improving skin hydration, brightness, and inflammation within a four-week observation period.
Patients and methods: Sixteen subjects with skin issues—including dryness, roughness, dull complexion, and enlarged pores—were recruited, with seven participants also presenting with chronic skin inflammation. Each subject received a single treatment using DermaShine PRO microneedling combined with HA35. Skin condition was evaluated 20 minutes post-treatment and during follow-up using a skin analyzer, an improved Visual Analog Scale (VAS), and patient satisfaction surveys.
Results: The results demonstrated significant improvement in skin dryness and roughness (P<0.01) and increased brightness (P<0.05) within 20 minutes of treatment. Subjects with chronic inflammation showed a marked reduction in inflammation hardness and erythema (P<0.01). The treatment effect persisted for over one month, with 100% of participants reporting satisfaction during the follow-up period. No adverse reactions related to the procedure or product were observed throughout the study.
Conclusion: Negative pressure microneedling combined with HA35 offers an effective, non-pharmaceutical approach for improving skin hydration, brightness, and mild to moderate inflammation within a short-term period. This treatment demonstrates sustained effects over the four-week observation period and maintains high patient satisfaction. Given its safety and non-invasive nature, it represents a promising option for aesthetic dermatology, with potential for further optimization in future studies.
DOI: 10.29245/2767-5092/2025/1.1191 View / Download PdfAnalysis of STAT3 Activation and Cytokine Production in Skin Cancer Patients
Skin cancer is a disease marked by the presence of abnormal cells within the skin tissues. These cells grow in a disorganized manner and reproduce uncontrollably at a rapid pace. Various factors contribute to the onset and progression of skin cancer; these can be genetic and environmental, psychological, microbiological, and immunological, such as inflammation and a weakened immune system. Signal transducers and activators of transcription (STATs) are a family of latent transcription factors activated in response to various cytokines and growth factors. This study aims to identify the association of STAT3 activation with pro-inflammatory cytokines such as IL-1 β, IL-6, and TNF-α. The phospo-STAT3/STAT3 ratio indicates significant activation levels of this transcription factor in skin cancer patients versus the control group. Also, we encountered higher serum concentrations of IL-1𝛽, IL-6, and TNF-𝛼 compared to the control group. Although our analysis did not show a correlation between STAT3 activation and cytokines, elevated levels of activated STAT3 offer new insights into the practical application of STAT3, which could help develop more effective treatments for this malignancy. In addition, the fact that patients show a higher serum concentration of cytokines highlights their key role in the pathophysiology of skin cancer.
DOI: 10.29245/2767-5092/2025/1.1197 View / Download Pdf